|
KMID : 0359319930330040711
|
|
Korean Journal of Veterinary Research
1993 Volume.33 No. 4 p.711 ~ p.717
|
|
|
Serum cortisol concentrations on the normal condition in Korean native goats by ELISA
|
|
|
|
|
Abstract
|
|
|
This study was performed to examine the charges of serum cortisol concentrations on the normal conditions in Korean native goats by enzyme-linked immunosorbent assay(ELISA).
The Korean native goats were 4 to 30 months old and weighted 6.0 to 28㎏. The goats were allowed ad libitum access to water and food.
To minimize the stress with handing and blood sampling, animals were adapted for 2 weeks before the experiment. After adaptation, intravenouse catheter was inserted left intact a jugular vein of 6 goats and blood samples were done 2∼3 days later. Experimental animals were divided into 2 groups which was non-catheterized and catheterized group in order to examine the effect for changes of serum concentration and circadian rhythm of cortisol.
The results were obtained as follows ;
The sensitivity of serum cortisol concentration was 20 pg/㎖. The intra-assay and inter-assay coefficient of variation were below 2.5% and 5%, respectively.
Serum concentration of cortisol was more higher in noo-catheterized group(32.84±16.78 ng/㎖) than catheterized group(23.20±10.29 ng/㎖). The difference according to months old in serum concentration of cortisol was the more higber on 4∼6 than 7∼12 and lowest over 12 months old.
A circadian rhythm in the serum concentration of cortisol in Korean native goats was not found significantly with 2 hours sampling intervals.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|